DEVELOPMENT OF FILM-FORMING HYDROGEL FORMULA OF 70% ETHANOL EXTRACT ELEUTHERINE BULBOSA (MILL.) URB. BULB AS A WOUND CARE MEDICATION

The plant Eleutherine bulbosa (Mill.) Urb. is empirically used by the community for wound healing. The wound-healing activity of the Eleutherine bulbosa (Mill.) Urb. plant is due to the influence of secondary metabolites, which act as antioxidants, anti-inflammatories, and antibacterials. This study aimed to develop a film-forming hydrogel formula as an alternative to conventional semisolid dosage forms, which has limitations in its application for wound healing. The design of this study used the simplex lattice design method to obtain the optimal glycerol-ethanol and polyvinyl alcohol(PVA)-carboxymethyl chitosan(CMCh) composition based on the parameters of pH, viscosity, and swelling ratio. Ethanol significantly influences the viscosity parameter and swelling ratio of the film-forming hydrogel (FFH) formula with 70% ethanol extract of Eleutherine bulbosa (Mill.) Urb. bulb 2.5%  at the optimal composition ratio of 0.835:0.165. Carboxymethyl chitosan significantly influenced the pH, viscosity, and swelling ratio parameters of the film-forming hydrogel (FFH) formula with 70% ethanol extract of Eleutherine bulbosa (Mill.) Urb. bulb 2.5%  at a composition ratio of 0.240:0.759.

The optimal film-forming hydrogel (FFH) formula with 70% ethanol extract of Eleutherine bulbosa (Mill.) Urb. bulb 2.5% is stable at storage for one month at 25°C ± 2°C with 60% ± 5% humidity based on parameter pH and swelling ratio. It has wound healing activity by reducing the wound area compared to the negative control.

Keywords : film-forming hydrogel, Eleutherine bulbosa (Mill.) Urb., simplex lattice design