The research aimed to determine the metabolite profile of the essential oil of Cinnamomum burmannii (Nees & T.Nees) Blume bark (C. burmannii bark oil) which has the potential as anti-inflammatory from 5 regions of Indonesia.
This study reports standardization of the bark of C. burmannii, analysis of the metabolite profile of the essential oil of the bark of C. burmannii, analysis of the composition of secondary metabolites using GC-MS, in vivo testing of anti• inflammatory effects using the paw test technique on Wistar rats with doses of 30, 60 and 90 mg/kg BW as well as the anti-inflammatory effect of essential oils from 5 regions in Indonesia with a dose of 60 mg/kg BW with indicators of edema inhibition percentage, number of inflammatory cells and TNF-a expression. Finally, an in silica test was carried out on the metabolite compounds. The results of this study indicate that the essential oil of the bark of C. burmanii from 5 regions in Indonesia has anti• inflammatory properties. The best is essential oil from Kerinci and Lombok Timur. Trans-Cinnamaldehyde can be used as an analytical marker because it was found in quite large amounts in all essential oils from 5 regions.
The results of the screening of metabolites that are predicted to have anti• inflammatory properties with molecular docking techniques namely: y-Muurolene, a• Terpineol, trans-Cinnamaldehyde, Copaene, trans-a-Bergamotene, Caryophylene, a• Muurulene, Caryophyllene oxide, D-Limonene, a-Pinene, Bornyl acetate and Linalool. Molecular docking with the target protein COX-1 a-Terpineol and with the target protein COX-2 y-Muurolene have the lowest binding affinity.
