The research aimed to determine the metabolite profile of the essential oil of Cinnamomum burmannii(Nees & T.Nees) Blume bark (C. burm a nni ibark oil) which has the potential as anti-inflammatory from 5 regions of Indonesia.
This study reports standardization of the bark of C. burm a nn i i ,analysis of the metabolite profile of the essential oil of the bark of C. bu rmannii , analysis of the composition of secondary metabolites using GC-MS, in v iv otesting of anti• inflammatory effects using the paw test technique on Wistar rats with doses of 30, 60 and 90 mg/kg BW as well as the anti-inflammatory effect of essential oils from 5 regions in Indonesia with a dose of 60 mg/kg BW with indicators of edema inhibition percentage, number of inflammatory cells and TNF-a expression. Finally, an i n s i l i c atest was carried out on the metabolite compounds. The results of this study indicate that the essential oil of the bark of C. bu r m a ni i from 5 regions in Indonesia has anti• inflammatory properties. The best is essential oil from Kerinci and Lombok Timur. Trans-Cinnamaldehyde can be used as an analytical marker because it was found in quite large amounts in all essential oils from 5 regions.
The results of the screening of metabolites that are predicted to have anti• inflammatory properties with molecular docking techniques namely: y-Muurolene, a• Terpineol, trans-Cinnamaldehyde, Copaene, trans-a-Bergamotene, Caryophylene, a• Muurulene, Caryophyllene oxide, D-Limonene, a-Pinene, Bornyl acetate and Linalool. Molecular docking with the target protein COX-1 a-Terpineol and with the target protein COX-2 y-Muurolene have the lowest binding affinity.