METABOLITE PROFILE OF Cinnamomum burmannii (Nees & T. Nees) Blume BARK ESSENTIAL OILS FROM 5 REGIONS IN INDONESIA AS A POTENT

The research aimed to determine the metabolite profile of the essential oil of Cinnamomum burmannii(Nees & T.Nees) Blume bark (C. burm a nni ibark oil) which has the potential as anti-inflammatory from 5 regions of Indonesia.

This study reports standardization of the bark of C. burm a nn i i ,analysis of the metabolite profile of the essential  oil of the bark of C.   bu rmannii ,  analysis of the composition of secondary metabolites using GC-MS, in v iv otesting of anti• inflammatory effects using the paw test technique on Wistar rats with doses of 30,  60 and 90 mg/kg BW as well as the anti-inflammatory effect of essential oils from 5 regions in  Indonesia  with  a  dose  of 60  mg/kg  BW  with  indicators  of edema  inhibition percentage,  number of inflammatory cells and TNF-a expression.  Finally,  an i n s i l i c atest was carried out on the metabolite compounds. The results of this study indicate that the essential  oil of the bark of C.   bu r m a ni i  from 5 regions  in Indonesia  has anti• inflammatory properties. The best is essential oil  from Kerinci and Lombok Timur. Trans-Cinnamaldehyde  can be used as an analytical marker because it  was found in quite large amounts in all essential oils from 5 regions.

The results of the screening of metabolites that are predicted to have anti• inflammatory properties with molecular docking techniques namely: y-Muurolene, a• Terpineol, trans-Cinnamaldehyde, Copaene, trans-a-Bergamotene, Caryophylene, a• Muurulene, Caryophyllene oxide, D-Limonene, a-Pinene, Bornyl acetate and Linalool. Molecular  docking  with the target  protein COX-1  a-Terpineol  and with the target protein COX-2 y-Muurolene have the lowest binding affinity.

Unduhan